{"id":406,"date":"2026-05-26T02:09:22","date_gmt":"2026-05-26T02:09:22","guid":{"rendered":"https:\/\/aviantestpro.com\/?p=406"},"modified":"2026-06-02T03:30:51","modified_gmt":"2026-06-02T03:30:51","slug":"rt-pcr%e6%b3%95%e3%81%a7%e9%b3%a5%e3%81%ae%e6%80%a7%e5%88%a5%e3%82%92%e8%aa%bf%e3%81%b9%e3%82%8b%e3%81%ab%e3%81%af%ef%bc%9f","status":"publish","type":"post","link":"https:\/\/aviantestpro.com\/ja\/how-do-we-test-the-birds-gender-using-rt-pcr-method\/","title":{"rendered":"RT-PCR\u6cd5\u3092\u7528\u3044\u3066\u9ce5\u306e\u6027\u5225\u3092\u5224\u5b9a\u3059\u308b\u65b9\u6cd5\uff08\u5b8c\u5168\u7248\u52d5\u753b\uff09"},"content":{"rendered":"<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-7387b849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<figure class=\"wp-block-embed is-type-video is-provider-youtube wp-block-embed-youtube wp-embed-aspect-16-9 wp-has-aspect-ratio\"><div class=\"wp-block-embed__wrapper\">\n<iframe loading=\"lazy\" title=\"\u30e9\u30dc\u306e\u65e5\u8ab2\u7fbd\u6bdb\u30b5\u30f3\u30d7\u30eb\u306eDNA\u691c\u67fb\uff5c\u9ce5\u306e\u6027\u5225\u8b58\u5225\u30d7\u30ed\u30bb\u30b9\" width=\"500\" height=\"281\" src=\"https:\/\/www.youtube.com\/embed\/Wkg0FkoDwfA?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe>\n<\/div><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-7387b849 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\">\n<p class=\"wp-block-paragraph\">\u3053\u306e\u30d3\u30c7\u30aa\u3067\u306f\u3001\u305d\u306e\u5168\u5bb9\u3092\u7d39\u4ecb\u3059\u308b\u3002<a href=\"https:\/\/aviantestpro.com\/ja\/%e9%b3%a5%e9%a1%9e%e6%80%a7%e5%88%a5dna%e6%a4%9c%e6%9f%bb\/\"> \u9ce5\u985e\u306eDNA\u9451\u5b9a<\/a> \u73fe\u4ee3\u306e\u691c\u67fb\u5ba4\u3067\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3002\u30d7\u30ed\u30bb\u30b9\u306f\u9069\u5207\u306a\u30b5\u30f3\u30d7\u30eb\u63a1\u53d6\u304b\u3089\u59cb\u307e\u308b\u3002\u5375\u80de\u306b\u306fDNA\u62bd\u51fa\u306b\u5fc5\u8981\u306a\u751f\u304d\u305f\u7d30\u80de\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u305f\u3081\u3001\u7121\u50b7\u306e\u5375\u80de\u3092\u542b\u3080\u65b0\u9bae\u306a\u7fbd\u6bdb\u3092\u614e\u91cd\u306b\u6e96\u5099\u3059\u308b\u3002\u5834\u5408\u306b\u3088\u3063\u3066\u306f\u3001DNA\u6fc3\u5ea6\u3092\u9ad8\u3081\u308b\u305f\u3081\u306b\u8840\u6db2\u30b5\u30f3\u30d7\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3082\u3042\u308b\u3002.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u63a1\u53d6\u5f8c\u3001\u691c\u67fb\u5ba4\u306f\u30b5\u30f3\u30d7\u30eb\u304b\u3089\u907a\u4f1d\u7269\u8cea\u3092\u5206\u96e2\u3059\u308b\u305f\u3081\u306bDNA\u62bd\u51fa\u3092\u884c\u3046\u3002\u8cea\u306e\u60aa\u3044DNA\u306fPCR\u5897\u5e45\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3057\u3001\u4e0d\u660e\u77ad\u306a\u7d50\u679c\u306b\u3064\u306a\u304c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001\u6b63\u78ba\u306a\u62bd\u51fa\u306f\u6975\u3081\u3066\u91cd\u8981\u3067\u3042\u308b\u3002\u7cbe\u88fd\u3055\u308c\u305fDNA\u304c\u5f97\u3089\u308c\u305f\u3089\u3001\u30b5\u30f3\u30d7\u30eb\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u3001\u9175\u7d20\u3001\u5897\u5e45\u8a66\u85ac\u3092\u542b\u3080PCR\u53cd\u5fdc\u7cfb\u306b\u79fb\u3055\u308c\u308b\u3002.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">PCR\u5897\u5e45\u4e2d\u3001\u9ce5\u985e\u306eDNA\u306e\u6a19\u7684\u9818\u57df\u306f\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u5185\u3067\u7e70\u308a\u8fd4\u3057\u30b3\u30d4\u30fc\u3055\u308c\u308b\u3002\u3053\u308c\u3089\u306e\u907a\u4f1d\u5b50\u30de\u30fc\u30ab\u30fc\u306b\u3088\u308a\u3001\u7814\u7a76\u5ba4\u306f\u9ce5\u306e\u30aa\u30b9\u307e\u305f\u306f\u30e1\u30b9\u306b\u95a2\u9023\u3059\u308b\u6027\u67d3\u8272\u4f53\u3092\u540c\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002\u5897\u5e45\u5f8c\u3001\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u3067DNA\u30d0\u30f3\u30c9\u3092\u53ef\u8996\u5316\u3059\u308b\u3002\u30d0\u30f3\u30c9\u306e\u30d1\u30bf\u30fc\u30f3\u3092\u5206\u6790\u3059\u308b\u3053\u3068\u3067\u3001\u6280\u8853\u8005\u306f\u305d\u306e\u9ce5\u304c\u30aa\u30b9\u304b\u30e1\u30b9\u304b\u3092\u5224\u65ad\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u3002.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u3053\u306e\u30d3\u30c7\u30aa\u306f\u307e\u305f\u3001\u6c5a\u67d3\u7ba1\u7406\u3001\u9069\u5207\u306a\u5b9f\u9a13\u5ba4\u3067\u306e\u53d6\u308a\u6271\u3044\u3001\u96fb\u6c17\u6cf3\u52d5\u7d50\u679c\u306e\u6b63\u78ba\u306a\u89e3\u91c8\u306e\u91cd\u8981\u6027\u3092\u5f37\u8abf\u3057\u3066\u3044\u308b\u3002\u5c0f\u3055\u306a\u624b\u9806\u306e\u30df\u30b9\u3067\u3055\u3048\u3001\u6700\u7d42\u7684\u306a\u7d50\u679c\u306b\u5f71\u97ff\u3092\u53ca\u307c\u3059\u53ef\u80fd\u6027\u304c\u3042\u308b\u3002\u3060\u304b\u3089\u3053\u305d\u3001\u4fe1\u983c\u6027\u306e\u9ad8\u3044\u9ce5\u985eDNA\u691c\u67fb\u306b\u306f\u3001\u5c02\u9580\u7684\u306a\u5b9f\u9a13\u5ba4\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\u4e0d\u53ef\u6b20\u306a\u306e\u3067\u3042\u308b\u3002.<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">PCR\u6cd5\u3092\u7528\u3044\u305f\u9ce5\u306e\u96cc\u96c4\u9451\u5225\u306f\u3001\u5f93\u6765\u306e\u96cc\u96c4\u9451\u5225\u6cd5\u306b\u4ee3\u308f\u308b\u79d1\u5b66\u7684\u304b\u3064\u975e\u4fb5\u8972\u7684\u306a\u65b9\u6cd5\u3067\u3042\u308b\u305f\u3081\u3001\u73fe\u5728\u3067\u306f\u30d6\u30ea\u30fc\u30c0\u30fc\u3001\u30da\u30c3\u30c8\u30aa\u30fc\u30ca\u30fc\u3001\u4fdd\u8b77\u30d7\u30ed\u30b0\u30e9\u30e0\u3001\u30ec\u30fc\u30b9\u9ce9\u611b\u597d\u5bb6\u306a\u3069\u306b\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u3002.<\/p>\n<\/div>\n<\/div>\n\n\n\n<p class=\"wp-block-paragraph\"><\/p>","protected":false},"excerpt":{"rendered":"<p>This video demonstrates the complete avian DNA sexing workflow used in modern laboratories. The process begins with proper sample collection. Fresh feathers containing intact follicles are carefully prepared because the follicle contains living cells needed for DNA extraction. In some cases, blood samples may also be used for higher DNA concentration. After collection, the laboratory performs DNA extraction to isolate genetic material from the sample. Accurate extraction is critical because poor-quality DNA can affect PCR amplification and lead to unclear results. Once purified DNA is obtained, the sample is transferred into a PCR reaction system containing primers, enzymes, and amplification reagents. During PCR amplification, target regions of bird DNA are [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":458,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[1],"tags":[],"class_list":["post-406","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-seno-avian-test"],"_links":{"self":[{"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/posts\/406","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/comments?post=406"}],"version-history":[{"count":2,"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/posts\/406\/revisions"}],"predecessor-version":[{"id":460,"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/posts\/406\/revisions\/460"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/media\/458"}],"wp:attachment":[{"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/media?parent=406"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/categories?post=406"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/aviantestpro.com\/ja\/wp-json\/wp\/v2\/tags?post=406"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}